A novel use of cholinesterase amperometric biosensor for selective determination of 2,4-dichlorophenoxyacetic acid
Mediantseva, E.P.; Vertlib, M.G.; Budnikov, G.K.; Tyshlek, M.P.
Prikladnaia Biokhimiia i Mikrobiologiia 34(2): 220-224
1998
ISSN/ISBN: 0555-1099 PMID: 9567297 Document Number: 487841
A new method of detection of 2,4-dichlorophenoxyacetic acid by means of immobilized monoclonal antibodies and an amperometric cholinesterase biosensor is proposed. A combination of monoclonal antibodies to 2,4-dichlorophenoxyacetic acid with an enzyme electrode provides high selectivity of measurements and allows trace amounts of this pesticide to be detected within a concentration range of 1 x 10(-1) to 5 x 10(-7) M with a sensitivity threshold of 5 x 10(-12) M.