Development of enzyme biosensor based on trypsin and conductometric thin-film electrodes for protein and artificial substrates determination
Biloivan, O.A.; Dziadevich, S.V.; Soldatkin, O.P.; Starodub, M.F.; Iel'ska, G.V.
Ukrainskii Biokhimicheskii Zhurnal 69(2): 14-18
1997
ISSN/ISBN: 0201-8470 PMID: 9463233 Document Number: 477525
Two analytical systems based on the soluble and immobilized trypsin and conductometric thin-film electrodes were developed to measure concentration of artificial substrate and protein in solution. It was shown that these systems allow one to determine concentrations of Ha-benzoyl-L-arginin-ethyl-ester in the range of 0.1-1.0 mM and concentrations of HSA: 0.1-2.0 mg/ml with soluble and 0.1-0.8 mg/ml with immobilized trypsin.