Development of conductometric biosensor based on alkaline phosphatase for determining concentration of cadmium ions
Sosovs'ka, O.F.; Berezhets'kyĭ, A.L.
Ukrains'kyi Biokhimichnyi Zhurnal 79(4): 102-109
2007
ISSN/ISBN: 0372-3909 PMID: 18219998 Document Number: 605627
The paper describes a novel conductometric biosensor sensitive to cadmium ions based on alkaline phosphatase immobilized on gold planar microelectrodes used as transducers. Assays have been carried out with paranitrophenyl phosphate as substrate for the immobilized enzyme. Various parameters such as reticulation time, along with pH, ionic strength and buffer concentration of the measuring solution were studied. The optimized biosensor was stable, reproducible and it exhibited a detection limit of 4.45 microM for cadmium ions.