Use of silver immunosalts for detecting HIV antibodies in microtiter plates
Poltavchenko, A.G.; Lavrinenko, I.A.; Kostrovskiĭ, V.G.; Tuzikov, F.V.; Karavaev, V.S.
Voprosy Virusologii 42(3): 120-123
1997
ISSN/ISBN: 0507-4088 PMID: 9297341 Document Number: 477345
The authors examined the possibility of replacing immunoperoxidase conjugates with silver sol (mean diameter of particles 9 nm) adsorption-bound to antispecies IgG in solid-phase enzyme immunoassay in microtitration plates. Experiments with commercial test systems Antigen and Recombitest-antiHIV-1,2 manufactured by the Vektor State Research Center of Virology and Biotechnology, Ministry of Health and Medical Industry of the Russian Federation, showed that the sensitivity of detecting anti-HIV with silver immunosols and boosting of the signal with physical development was no less sensitive than standard EIA with immunoperoxidase conjugate. Preparation of silver immunosols is simple, easily reproducible, and is based on sparing and economic use of immunoreagents.