Binding characteristics of bovine serum albumin-aflatoxin B1 to polystyrene microtiter plates: importance of hapten to carrier protein molar ratio
Vyjayanthi, V.; Capoor, A.K.; Sashidhar, R.B.
Indian Journal of Experimental Biology 33(5): 329-332
1995
ISSN/ISBN: 0019-5189 PMID: 7558191 Document Number: 848
Binding characteristics of bovine serum albumin-aflatoxin B-1 conjugates with high (1:54), medium (1:25) and low (1:9) hapten to carrier molar ratios, to the polystyrene microtiter plates are influenced by the stoichiometry of the hapten (Aflatoxin B-1) to the carrier protein (bovine serum albumin). Conjugates with optimal hapten to carrier molar ratios (1:25) showed a better binding capacity to the polystyrene microtiter plate as compared to the conjugates with the high molar ratios in a non-competitive ELISA for aflatoxin B-1. Denaturation of the conjugate with molar ratio of 1:54 in order to enhance its binding capacity, however, did not result in any significant improvement.
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