Loss of high-molecular-weight proteins in liver microsomes of rats treated with hepatocarcinogens
Khongwir, B.S.; Dani, H.M.; Dhanda, J.S.
Journal of Environmental Pathology Toxicology and Oncology Official Organ of the International Society for Environmental Toxicology and Cancer 16(1): 27-31
1997
ISSN/ISBN: 0731-8898 PMID: 9256929 Document Number: 475882
Microsomal preparations from rats treated with 2-acetylaminofluorene (2-AAF) or with 4-dimethylaminoazobenzene (4-DAB), alone or followed by phenobarbital (PB), showed almost complete loss of microsomal proteins of molecular weights higher than 60,000, as shown by Sephadex G-150 gel filtration technique. Induction of a number of microsomal proteins in the lower ranges of molecular weights was also recorded due to the treatment of these two hepatocarcinogens with and without the use of PB as a promoter. These modifications in the protein patterns of microsomes might be due to altered genetic expression resulting in uncontrolled cell division/cell cycle.