Determination of molecular weight and related hydrodynamic parameters of high molecular weight protein fraction from a few oilseeds
Murty, B.S.; Prakash, V.; Rao, M.S.
Indian Journal of Biochemistry and Biophysics 27(5): 295-299
1990
ISSN/ISBN: 0301-1208 PMID: 2079335 Document Number: 361081
The hydrodynamic parameters of the major protein fraction, viz. arachin from groundnut, .alpha.-globulin from sesame seed, brassin (M) from mustard seed and helianthinin from sunflower seed, have been determined in a single solvent system (0.05 M Tris-HCl buffer, pH 7.5 containing 0.5 M sodium chloride): sedimentation coefficient (s20,w0) and diffusion coefficient (D20,w0) by analytical ultracentrifugation, intrinsic viscosity [.eta.] by Ostwald viscometry and partial specific volume (.hivin.V) by densimetry. The molecular weights (M) of the four proteins, calculated using the sedimentation-viscosity and sedimentation-diffusion coefficient methods, were found close to each other. The values have been compared with those in the literature and the reasons for discrepancies have been discussed.