Determination of protein concentrations and their molecular weight in tears from cats with normal corneas and cats with corneal sequestrum

Davidson, H.J.; Gerlach, J.A.; Bull, R.W.

American Journal of Veterinary Research 53(10): 1756-1759

1992


ISSN/ISBN: 0002-9645
PMID: 1456514
Document Number: 395938
Protein concentration was determined, using the Bradford technique, in tears from cats with normal corneas and from cats with corneal sequestrum. Tears from the former group contained 5.81 +/- 2.29 mg of protein/ml; those from corneal sequestrum-affected cats contained 6.21 +/- 2.21 mg/ml. Difference between the 2 values was not significant. Molecular weight determination was made, using 4 to 20% sodium dodecyl sulfate-polyacrylamide gels. Molecular mass of proteins ranged from 263 to 14 kDa. There was no detectable difference in the band patterns for the 2 groups.

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