Temperature-induced expression of human-mouse chimeric Fab
Zan, H.; Yeh, M.
Chinese Journal of Biotechnology 13(2): 63-70
1997
ISSN/ISBN: 1042-749X PMID: 9343703 Document Number: 474159
The temperature-induced expression vector pHZ01 with lambda PRPL promoter for the efficient expression of human-mouse chimeric Fab was constructed. Three kinds of chimeric Fab were expressed in E. coli: anti-prostate specific antigen (PSA) chimeric Fab, anti-lysozyme (HEL) chimeric Fab, and anti-tetanus toxoid (TT) chimeric Fab. All the soluble chimeric Fabs expressed showed specific antigen-binding activities.
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