Neoplasm-inhibiting effect and sensitivity-promoting effect of indomethacin in vitro
Zhang, G.; Duan, C.; Shi, J.; Leng, A.
Hunan Yi Ke Da Xue Xue Bao 22(6): 478-482
1997
ISSN/ISBN: 1000-5625 PMID: 10072983 Document Number: 471098
Objective: To explore an anti-tumor effect of indomethacin on human colon adenocarcinoma cell line HCT116 and its mechanism. Methods: MTT, agarose get electrophoresis, TdT and flow cytometry were used to study cell proliferation and apoptosis. Results: Indomethacin significantly inhibited the proliferation of HCT116 cells and induced apoptosis with a "dose-time' dependent pattern. A synergic effect of inhibiting cancer cells proliferation was observed when combined with 5-Fu. Conclusion: Inhibition of proliferation and induction of apoptosis contribute to the mechanisms for anti-tumor activity of indomethacin.