Polymorphisms of uncoupling protein (UCP) and beta 3 adrenoreceptor genes in obese people submitted to a low calorie diet
Fumeron, F.; Durack-Bown, I.; Betoulle, D.; Cassard-Doulcier, A.M.; Tuzet, S.; Bouillaud, F.; Melchior, J.C.; Ricquier, D.; Apfelbaum, M.
International Journal of Obesity and Related Metabolic Disorders Journal of the International Association for the Study of Obesity 20(12): 1051-1054
1996
ISSN/ISBN: 0307-0565 PMID: 8968848 Document Number: 467011
OBJECTIVE: To investigate whether the genetic polymorphisms of the uncoupling protein (UCP) and beta3 adrenergic receptor (beta3 AR) were associated with differences of weight loss in obese patients submitted to a low calorie diet. DESIGN: Longitudinal, clinical intervention study of a 25% restriction in energy intake with respect to genotypes. SUBJECTS: 163 patients with a body mass index above 27. MEASUREMENTS: Body weight and body mass index at baseline and after 2.5 months, genotypes by polymerase chain reaction followed by enzymatic digestion. RESULTS: For the UCP polymorphism, two alleles, 1 and 2 were identified with respective frequencies of 0.27 and 0.73. The allele 1 was associated with lower body weight loss after diet: 4,6,5.7 and 7.1 kg for the 1-1, 1-2 and 2-2 genotypes respectively (P less than 0.05). No difference in weight loss was found according to the beta3 AR Trp64Arg mutation. CONCLUSIONS: A genetic variant of the UCP gene is associated with a resistance to low calorie diet. This result, together with previous data on body weight gain, supports the hypothesis of a role of UCP and brown adipose tissue in the body weight regulation in humans. The importance of the Trp64Arg mutation of the beta3 AR in the resistance to low calorie diet is still to demonstrate.