Protective effects of tetrandrine on CCl4-injured hepatocytes
Chen, X.H.; Hu, Y.M.; Liao, Y.Q.
Zhongguo Yao Li Xue Bao 17(4): 348-350
1996
ISSN/ISBN: 0253-9756 PMID: 9812721 Document Number: 466813
AIM: To study the protective effects of tetrandrine (Tet) on CCl-4-injured hepatocytes. METHODS: The cultured rat liver cells were poisoned by CCl-4 (10 mmol cntdot L-1). The membrane fluidity was detected by 1,6-diphenyl-1,3,5-hexatriene (DPH), a lipid probe. The Ca-2+ concentration was assayed with Fura 2-AM, a sensitive calcium indicator. RESULTS: Tet (1-1000 nmol cntdot L-1) increased viability of liver cell (from 71% to 72%-89%), reduced lactate dehydrogenase (LDH) release, and malondialdehyde (MDA) formation. Tet prevented the heightening of the intracellular Ca-2+ concentration and the attenuation of the membrane fluidity of liver cells (P lt 0.05). CONCLUSION: Tet had a protective effect on CCl-4-injured hepatocytes by inhibiting the lipid peroxidation, improving the membrane fluidity, and lessening the Ca-2+ concentration.