Polarization fluoroimmunoassay of propazine in reversed micelles of aerosol OT in octane
Matveeva, E.G.; Samsonova, Z.V.; Eremin, S.A.
Bioorganicheskaia Khimiia 22(12): 931-937
1996
ISSN/ISBN: 0132-3423 PMID: 9054343 Document Number: 456371
The interaction between fluorescein-labeled propazine and antibodies against this hapten was studied in the reversed micelles of Aerosol OT in n-octane by a polarization fluoroassay. The effect of the hydration degree of micelles W-0 (W-0 = (H-2O)/(Surf)), which determines their size and surfactant concentration, on the binding of the antigen with antibodies was studied. A high hydration degree of the reversed micelles (W-0 = 15-30) and low concentration of the surfactant (less than 50 mM) are optimal for binding. The binding efficacy depends upon the structure of the fluorescein-labeled hapten, particularly upon the length of the bridge binding fluorescein with propazine. It was shown that the polarization fluoroimmunoassay of propazine may be carried out in a reversed micellar system in nonpolar organic solvent (octane) with a detection limit of about 100 nM (20 mu-g/l). This is an order of magnitude higher than that achievable upon analysis in aqueous medium. The proposed polarization fluoroimmunoassay in a reversed micellar system makes it possible to detect haptens that are poorly soluble in water directly in organic extracts, e.g., in chloroform solutions.