Catalytic properties of the membrane enzyme prostaglandin H synthetase in a system of inverted micelles of aerosol OT in octane
Mevkh, A.T.; Sud'ina, G.F.; Lagutina, I.O.; Levashov, A.V.
Biokhimiia 50(10): 1719-1723
1985
ISSN/ISBN: 0320-9725 PMID: 2416356 Document Number: 244429
Prostaglandin H synthetase solubilized in octane with the aid of hydrated reversed micelles of Aerosol OT (AOT) exhibits a catalytic activity dependent on hydration of the surface-active substance and its concentration. The maximum rate of the reaction is attained at the H2O/AOT molar ratio equal to 20 and amounts to a value close to the one observed in an aqueous solution. The inactivation rate of the enzyme in the course of the reaction does not depend on the water content in the system and is described by a Kin value commensurable with the Kin in an aqueous solution.