Top Abstract Purchase Related
Home
>
Node
>
Subnode

Beta-endorphin: photoaffinity labelling of a non-opioid binding site in a human neuroblastoma

Hammonds, R.G.; Westphal, M.; Li, C.H.

Biochemical and Biophysical Research Communications 128(3): 1310-1314

1985

ISSN/ISBN: 0006-291X
PMID: 2988533
Document Number: 244430
A photoaffinity reagent 2-nitro-4-azidophenylsulfenyl chloride (2,4-NAPS-Cl) and synthetic analogs of human .beta.-endorphin (.beta.h-EP) were employed to demonstrate the presence of receptor sites specific for .beta.h-EP but of non-opioid character in a human neuroblastoma cell line (IMR-32). The radioactive photoaffinity probe was carried out using [125I-Tyr1,2,4-NAPS-Trp27]-.beta.h-EP and IMR-32 cell membranes. After solubilization with sodium dodecyl sulfate (SDS) and SDS polyacrylamide gel electrophoresis, a single labeled protein band was identified with a MW of 72,000. Labeling was blocked by .beta.h-EP or .beta.h-EP-(6-31) but remained in the presence of .beta.h-EP-(1-27). The specificity of this band is identical to that of the non-opioid site previously characterized. Various nonionic or zwitterionic detergents did not extract the labeled non-opioid site.

Document emailed within 1 workday
Secure & encrypted payments

Schoffelmeer, A.N.; Yao, Y.H.; Gioannini, T.L.; Hiller, J.M.; Ofri, D.; Roques, B.P.; Simon, E.J. 1990: Cross-linking of human [125I]beta-endorphin to opioid receptors in rat striatal membranes: biochemical evidence for the existence of a mu/delta opioid receptor complex Journal of Pharmacology and Experimental Therapeutics 253(1): 419-426
Johnson, L.W.; Smith, C.H. 1982: Identification of the glucose transport protein of the microvillous membrane of human placenta by photoaffinity labelling Biochemical and Biophysical Research Communications 109(2): 408-413
Benavides, J.; Dubois, A.; Dennis, T.; Hamel, E.; Scatton, B. 1989: Omega 3 (peripheral type benzodiazepine binding) site distribution in the rat immune system: an autoradiographic study with the photoaffinity ligand [3H]PK 14105 Journal of Pharmacology and Experimental Therapeutics 249(1): 333-339
Laxmi, N.A.; Vijayan, E. 1998: Effects of chronic systemic administration of opioid peptides, naloxone and N-acetyl beta-endorphin antiserum on gonadotropins and testicular functions in the rat Indian Journal of Experimental Biology 36(4): 361-366
Lin, J.; Lu, G.; Weng, J. 1994: Effects of beta-endorphin on phytohemagglutinin-induced lymphocyte proliferation and mouse plaque-forming cell response via an opioid receptor mechanism Chinese Medical Sciences Journal 9(4): 245-247
Moore, G.J.; Pollock, B.; Ganter, R.; Franklin, K. 1990: Evaluation of azidobenzoyl and benzoylbenzoyl groups for photoaffinity labelling of angiotensin receptors in responding tissues Proceedings of the Western Pharmacology Society 33: 261-264
Bryant, S.D.; Attila, M.; Salvadori, S.; Guerrini, R.; Lazarus, L.H. 1994: Molecular dynamics conformations of deltorphin analogues advocate delta opioid binding site models Peptide Research 7(4): 175-184
Sheng, Y.; Sali, A.; Herzog, H.; Lahnstein, J.; Krilis, S.A. 1996: Site-directed mutagenesis of recombinant human beta 2-glycoprotein i identifies a cluster of lysine residues that are critical for phospholipid binding and anti-cardiolipin antibody activity Journal of Immunology 157(8): 3744-3751
Burbach, J.P.; Loeber, J.G.; Verhoef, J.; Wiegant, V.M.; de Kloet, E.R.; de Wied, D. 1980: Selective conversion of beta-endorphin into peptides related to gamma- and alpha-endorphin Nature 283(5742): 96-97
Liu-Chen, L.Y.; Li, S.X.; Tallarida, R.J. 1990: Studies on kinetics of [3H]beta-funaltrexamine binding to mu opioid receptor Molecular Pharmacology 37(2): 243-250
Jenkins, B.G.; Lauffer, R.B. 1990: Detection of site-specific binding and co-binding of ligands to human serum albumin using 19F NMR Molecular Pharmacology 37(1): 111-118
Werling, L.L.; Brown, S.R.; Puttfarcken, P.; Cox, B.M. 1986: Sodium regulation of agonist binding at opioid receptors. II. Effects of sodium replacement on opioid binding in guinea pig cortical membranes Molecular Pharmacology 30(2): 90-95
Liotta, A.S.; Houghten, R.; Krieger, D.T. 1982: Identification of a beta-endorphin-like peptide in cultured human placental cells Nature 295(5850): 593-595
Lypka, A.; Lazowski, J.; Szczudlik, A.; Wasek, Z. 1983: The depot-form of glucocorticoid decreases cortisol but not ACTH and beta-endorphin levels in human plasma Hormone and Metabolic Research 15(2): 104-106
Imashuku, S.; Fossett, M.C.; Green, A.A. 1979: Characterization of adenosine cyclic 3':5'-monophosphate-binding proteins in human neuroblastoma Cancer Research 39(8): 3006-3013
Edwards, R.A.; Cupples, C.G.; Huber, R.E. 1990: Site specific mutants of beta-galactosidase show that Tyr-503 is unimportant in Mg2+ binding but that Glu-461 is very important and may be a ligand to Mg2+ Biochemical and Biophysical Research Communications 171(1): 33-37
Koopmann, W.; Ediriwickrema, C.; Krangel, M.S. 1999: Structure and function of the glycosaminoglycan binding site of chemokine macrophage-inflammatory protein-1 beta Journal of Immunology 163(4): 2120-2127
Celio, M.R.; Höllt, V.; Buetti, G.; Pasi, A.; Bürgisser, E.; Eberle, A.; Kopp, G.; Siebenmann, R.; Friede, R.L.; Landolt, A.; Binz, H.; Zenker, W. 1980: Immunohistochemical study of beta-endorphin and related peptides in the "invading cells" of the human neurohypophysis during ontogenesis and adulthood Advances in Biochemical Psychopharmacology 22: 271-283
Toselli, M.; Tosetti, P.; Taglietti, V. 1997: Mu and delta opioid receptor activation inhibits omega-conotoxin-sensitive calcium channels in a voltage- and time-dependent mode in the human neuroblastoma cell line SH-SY5Y Pflugers Archiv: European Journal of Physiology 433(5): 587-596
Deitch, E.A.; Xu, D.; Bridges, R.M. 1988: Opioids modulate human neutrophil and lymphocyte function: thermal injury alters plasma beta-endorphin levels Surgery 104(1): 41-48