Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination
Hang, J-feng.; Wu, Y-song.; Xu, W-wen.; Yu, W-hong.; Huang, Y.; Li, M.
Zhonghua Zhong Liu Za Zhi 29(3): 189-192
2007
ISSN/ISBN: 0253-3766 PMID: 17649634 Document Number: 612950
Objective To prepare a rapid and sensitive diagnostic kit for detection of CA50 based on time-resolved fluoroimmunoassay. Methods A sandwich-TRFIA diagmostic kit was developed using anti-CA50 monoclonal antibody and all parameters of the kit were evaluated. Results The linear measurement range of the kit was (5 similar to 300) U/ml. The sensitivity was 0. 2 U/ml. The intra- and inter-assay coefficients of variation were 4. 3% similar to 8. 2% and 7. 7% similar to 11. 2%, respectively. There was no cross-reaction with CEA, CA12-5, CA15-3 and AFP. The cross reactivity with CA19-9 was 0. 7 U/ml. The correlation coefficient of detection results of 107 blood samples between this newly developed kit and commercially available CA50 RIA kit was 0. 901. Conclusion This newly developed CA50-TRFIA kit is a valuable test tool for clinical application with even better sensitivity, specificity and accuracy.