Rapid time-resolved fluoroimmunometric analysis of myoglobin
Gracheva, E.A.; Nechaeva, M.V.; Roshke, V.V.; Rykova, E.I.; Shishkin, G.V.; Bredikhin, A.V.
Voprosy Meditsinskoi Khimii 37(5): 89-92
1991
ISSN/ISBN: 0042-8809 PMID: 1759412 Document Number: 378311
High performance quantitative procedure for evaluation of myoglobin in blood serum is developed. The principle of two-site immunometry was used involving monoclonal antibodies against nonoverlapping epitopes of myoglobin molecule. The fluorescent compound containing europium served as a label of the antibody. Modification of the single-step assay enabled to increase the velocity of the assay to about 30 min and simplify it. The assay allowed estimation of myoglobin over a broad range of concentrations from 2 ng up to 1,000 ng/ml. The assay may be recommended for use in clinical practice for express diagnosis of myocardium infarction.