Rapid engraftment of peripheral blood progenitor cell grafts purged with B cell-specific monoclonal antibodies and immunomagnetic beads
Dreger, P.; von Neuhoff, N.; Suttorp, M.; Löffler, H.; Schmitz, N.
Bone Marrow Transplantation 16(4): 627-629
1995
ISSN/ISBN: 0268-3369 PMID: 8528184 Document Number: 452977
PBPC harvesting was performed in two patients with advanced-stage low-grade non-Hodgkin's lymphoma after mobilization with dexa-BEAM chemotherapy plus G-CSF. The collected grafts were subjected to immunomagnetic purging using B cell-specific moAbs and paramagnetic beads. Immunophenotypic and/or molecular analysis of the resulting products (PCR amplification of t(14;18) or CDR-111 rearrangements) demonstrated successful depletion of lymphoma cells. Rapid and durable hematopoietic recovery occurred after reinfusion of the purged grafts following myeloablative radiochemotherapy (9-10 days to neutrophils gt 0.5 times 10-9/l; 9-11 days to platelets gt 20 times 10-9/l). We conclude that effective immunomagnetic purging of PBPC grafts is feasible without affecting engraftment.