Hematopoietic progenitor cell counts performed by the Sysmex SE-9000 analyzer can guide timing of peripheral blood stem cell harvest
Endoh, A.; Yagihashi, A.; Asanuma, K.; Moriai, R.; Izawa, A.; Koyanagi, Y.; Sato, T.; Kobayashi, D.; Watanabe, N.
Anticancer Research 21(1b): 601-604
2001
ISSN/ISBN: 0250-7005 PMID: 11299812 Document Number: 528103
Background: We studied whether the hematopoietic progenitor cell (HPC) count in peripheral blood as evaluated by an automated counter, the Sysmex SE-9000, correlated with CD34 positive (+) cell count and therefore could guide the timing of peripheral blood stem cell (PBSC) harvest. Materials and Methods: HPC count and flow cytometric CD34+ cell count were measured in 90 peripheral blood samples and 30PBSC samples. The correlation between HPC count and apheretic CD34+ cell yield was examined in 19 patients. Results: HPC count showed good correlations with CD34+ cell count in peripheral blood (r=0.699) and PBSC (r=0.892). The correlation between peripheral blood HPC count and apheretic CD34+ cell yield also was good (r=0.789). Conclusion: Automated HPC counting can be used as a screening test to guide the timing of PBSC harvest.