Simplified enzyme-linked immunoadsorbent assays for myelin basic protein and antibodies to myelin basic protein
Chen, J.; Wang, R.; Li, C.; Xiong, C.; Wang, D.; Lin, J.; Zhang, Q.; Xu, J.; Yao, J.; Ling, L.
Hua Xi Yi Ke Da Xue Xue Bao 26(2): 125-130
1995
ISSN/ISBN: 0257-7712 PMID: 7490014 Document Number: 449135
Synchronous quantitative determination using simplified enzyme-linked immunoadsorbent, assay has been developed for myelin basic protein (MBP) and antibodies to myelin basic protein (Anti-MBP). Antisera were prepared by immunizing rabbits with purified MBP from human white matter. Antibodies to MBP was conjugated with horseradish peroxidase (HRP) by using modified periodate oxidation method. Minimum detection amounts of MBP and Anti-MBP are 0.25 ng/ml and 0.20 ng/ml respectively. The activities of Anti-MBP-HAP P conjugates are stable (stored at -30 degree C) for at least 2 years. The detection procedure can be accomplished within 24 hours.