The isolation and purification of the protective antigen and the edema factor from the culture filtrate of Bacillus anthracis STI-1
Rogov, S.N.; Beznosov, M.V.; Chugaĭ, V.L.; Bashkova, V.A.
Meditsinskaia Parazitologiia i Parazitarnye Bolezni 4: 35-38
1995
ISSN/ISBN: 0025-8326 PMID: 8587516 Document Number: 449038
A rapid method for producing a protective antigen (PA) and edema factor (EF), components of anthrax toxin, is described. The specific features of the method are as follows: addition of a mixture of protease inhibitors to the culture fluid; simultaneous concentration on a fiber filter and adsorption on hydroxylapatite, followed by non-linear gradient a phosphate concentration; purification of elution of a phosphate concentration; purification of eluates from salts via electrodialysis. Resultant from protein desorption, PA and EF agents are electrophoretically homogeneous by no less than 80%, 10 liters of filtrate yielded 8 mg of PA and 5 mg of EF. The resultant agents were tested for their biological properties and protective activity.