Isolation, purification, and characteristics of the new restriction enzymes BciBI and BciBII, produced by Bacillus circulans
Samko, O.T.; Kalugin, A.A.; El'darov, M.A.; Karpychev, I.V.; Anikeĭcheva, N.V.; Khoroshutina, E.B.; Skriabin, K.G.; Librik, G.I.; Sokolov, N.N.
Bioorganicheskaia Khimiia 20(12): 1327-1333
1994
ISSN/ISBN: 0132-3423 PMID: 7695650 Document Number: 429816
New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification--by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.