A method for immunoisolation of sporocysts of Schistosoma mansoni in nonsusceptible snails
Sullivan, J.T.; Hu, P.C.
Journal of Parasitology 81(6): 1029-1031
1995
ISSN/ISBN: 0022-3395 PMID: 8544049 Document Number: 448643
A technique is described for immunoisolation of sporocysts of Schistosoma mansoni in nonsusceptible Biomphalaria glabrata by microencapsulation in agarose. Based on histological evidence, all 11 microencapsulated sporocysts implanted into 3 schistosome-resistant 13-16-R1 snails were alive at 72 hr postimplantation, although in a developmentally retarded condition. However, among 146 sporocysts derived from miracidia that had penetrated 5 snails, 96% were dead by 72 hr. These results suggest that hemocyte contact is necessary for rapid sporocyst death in vivo.