Studies on common antigenicities between the Belo Horizonte strain, Brazil of Schistosoma mansoni eggs and Biomphalaria snails by immunoelectrophoresis
Iwanaga, Y.; de Santana, J.V.; Gonçalves, J.F.
Southeast Asian Journal of Tropical Medicine and Public Health 23(1): 98-102
1992
ISSN/ISBN: 0125-1562 PMID: 1381846 Document Number: 388789
Immunoelectrophoretic studies of common antigenicities were carried out by using rabbit sera immunized against the Belo Horizonte strain of S. mansoni eggs and crude antigens of Biomphalaria snails and vice versa. With regard to common antigenicities between S. mansoni eggs and Biomphalaria snails, S. mansoni eggs produced 4 to 5 bands with B. glabrata pigmentado, 3 to 4 bands with B. glabrata albino and only 1 band with B. straminea. In the laboratory, the infection rate of S. mansoni miracidia to B. glabrata pigmentado was 64.3% and 55.0% for B. glabrata albino, but B. straminea was not found to be susceptible to S. mansoni miracidia. It was observed that more bands were seen between S. mansoni egg and suitable hosts. Biomphalaria snail crude antigens were fractionated by Sephadex G-100 column, and each fraction antigen was tested with anti-S. mansoni egg sera by immunoelectrophoresis. As results, 3 fractions were collected from each snail strain. The common antigenicities between fractionated antigens from Biomphalaria snail crude antigens and anti-S. mansoni egg sera mostly existed in the first fraction, which were estimated to have MWs over 45 000.