Saltatory movements of the cytoplasmic granules in the cells of an ESK culture
Speranskaia, S.R.; Votchal, M.S.; Vorob'ev, I.A.
Tsitologiia 37(8): 783-790
1995
ISSN/ISBN: 0041-3771 PMID: 8669128 Document Number: 447580
Saltatory movements of large (0.3-0.8 micron) granules in the cytoplasm of PK cells were described in norm and after nocodazole and sodium azide treatments. In untreated cells the length of single movements was up to 2 micron and even more (sometimes up to 5 micron). Nocodazole at a concentration of 0.2 micron and sodium azide at a concentration of 20 micron inhibited all rapid movements longer than 1.2 micron, but did not affect the frequency of movements shorter than 1 micron. The effect of nocodazole was reversible: the long rapid movements were seen resumed after its removal. A comparison of histograms (length versus frequency) of the rapid movements of granules in norm and after nocodazole and sodium azide treatments made it possible to conclude that real saltations (ATP and microtubule-dependent motions) are rapid translocations which exceed 1 micron.