Calibration and standardization in enzyme immunoassay
Kuwa, K.
Nihon Rinsho. Japanese Journal of Clinical Medicine 53(9): 2232-2238
1995
ISSN/ISBN: 0047-1852 PMID: 7474386 Document Number: 445361
Calibration is performed using multipoints of the calibrator in enzyme immunoassay (EIA). Calibration curves in EIA are frequently represented by non-linearity. Therefore, calibration in EIA is fitted with suitable functions. These functions are demonstrated with logistic curve, logit-log conversion, spleen function, etc. Consequently, the calibration procedure is indicated for the manufacturer's instruction, and also the calibrator is prepared as a commercial available reagent kit. To minimize EIA measurements among laboratories, and among methods, standardization with a common suitable reference material is needed. Reference materials for EIA, international standards are prepared by the expert committee of WHO. These standards must be transferred to national standards, manufacturer's standards and calibrators of commercial available reagent kits. Finally, the measured value from the routine method is maintained with commutability and traceability.