Enzyme immunoassay for staphylococcal enterotoxin a
Kauffman, P.E.
Journal - Association of Official Analytical Chemists 63(5): 1138-1143
1980
ISSN/ISBN: 0004-5756 PMID: 6997261 Document Number: 155677
An enzyme immunoassay procedure specifying alkaline phosphatase-labeled enterotoxin A was used to determined enterotoxin in standardized solutions and food extracts. Staphylococcus aureus Cowan 1 cells were used to separate unbound from antibody-bound enterotoxin. The method is sensitive to 2 ng enterotoxin A/mL; cross reactions with other staphylococcal enterotoxins did not interfere with the specificity. The method is sensitive and precise enough to serve as a reliable alternative to radioimmunoassay for enterotoxin A.