Detection of 54-kDa protein overexpressed by chloroquine-resistant Plasmodium berghei ANKA strain in pyronaridine-resistant P berghei ANKA strain
Li, G.D.; Liu, S.Q.; Ye, X.Y.; Qu, F.Y.
Zhongguo Yao Li Xue Bao 16(1): 17-20
1995
ISSN/ISBN: 0253-9756 PMID: 7771189 Document Number: 442675
Immunoblotting analysis and immunoelectron microscopy were used to detect the 54-kDa protein, expressed in chloroquine-resistant (CR) Plasmodium berghei, in pyronaridine-resistant (PR) P. berghei ANKA strain. PR parasites, like CR parasites, mainly overexpressed 2 major bands of 37 (36-38) kDa and 16 (15-17) kDa which were considered to be 2 subunits of 54 (52-62) kDa protein. Three of 7 times of experiments showed a 54-kDa and a 96 (95-100) kDa bands. The proteins were predominantly localized in the cytoplasm of trophozoites, schizonts, and merozoites of erythrocytic stage of P. berghei although some were distributed in cytoplasm of erythrocytes infected with parasites. Both PR and CR parasites overexpressed the same proteins.