Long-term preservation of streptococci
Brahmadathan, K.N.; Pandian, R.; Koshi, G.
Indian Journal of Medical Research 101(5): 64-65
1995
ISSN/ISBN: 0019-5340 PMID: 7729845 Document Number: 439834
Strains of 12 T types of group A streptococci preserved by sand desiccation and stored at 4 degrees to 10 degrees C were regularly sub-cultured to check their viability. For this, streptococci coated onto sand particles mixed with sterile sheep blood were inoculated into Todd-Hewitt broth with added blood and incubated for 24 to 48 h. Checking for viability every six months showed that group A streptococci could be preserved by this method for 21 yr. Our study shows that sand desiccation is a convenient and cheap method for the long-term preservation of streptococci in laboratories where other methods of preservation are not feasible on a regular basis.