The mechanisms of the joint effect of chorionic gonadotropin and recombinant interleukin-2 on the splenocytes during the formation of a humoral immune response
Shirshev, S.V.
Tsitologiia 36(5): 459-464
1994
ISSN/ISBN: 0041-3771 PMID: 7809982 Document Number: 437763
In the spleen cell culture of female mice (CBA x C57BL/6)F1, transduction mechanisms of associative effect of chorionic gonadotropin (CG) and recombinant interleukin 2 (IL-2) have been investigated. The functional activity of splenocytes was estimated in the syngeneic transfer system, according to the number of plaque forming cells. It has been established that a 1 hour incubation of CG (40-200 IU) with intact spleen cells in vitro suppresses their ability to form the adoptive immune response. On the contrary, the common hormone effect with IL-2 (150 IU) increases more than twofold the plaque forming cell number. The costimulating effect of hormone is dose-dependent and is revealed only upon utilization of a high CG concentration, while in a low concentration and on the background of IL-2, the hormone completely loses its independent immunodepressive effect. During addition of cells, inhibiting the secondary messengers formation, to the culture it has been determined that the CG-dependent costimulation is completely lost during the blockade of "slow" Ca(2+)-canals, and partly lowered during the inhibition of phosphatidylinositide resynthesis processes. The hormonal and interleukin signal transduction in their common effect does not depend on prostaglandin synthesis. The independent immunostimulating IL-2 effect is displayed only on the background of blockade of cyclooxygenase enzymes or inositol-1-monophosphatase.