The mouse 5-HT2B receptor: possible involvement in trophic functions of serotonin
Choi, D.S.; Colas, J.F.; Kellermann, O.; Loric, S.; Launay, J.M.; Rosay, P.; Maroteaux, L.
Cellular and Molecular Biology 40(3): 403-411
1994
ISSN/ISBN: 0145-5680 PMID: 7920184 Document Number: 436327
The novel serotonin receptor 5-HT-2B shows the highest homology to the 5-HT-2 family of receptors. The pharmacological profile of membranes from 5-HT-2B cDNA stably transfected LMTK-cell line, corresponds to a new 5-HT-2-like receptor named 5-HT-2B although some difference exists between the mouse and rat pharmacology. A similar pharmacological profile is detected on the immortalized teratocarcinoma-derived cell line 1C11 upon 2 days of serotoninergic differentiation by cAMP. In both cell lines, the analysis 125I-DOI binding reveals the presence of a single class of sites, the affinity of which is one order of magnitude lower than the one reported for the 5-HT-2A receptor. This demonstrates that the 5HT-2b receptor is functionally expressed before the complete serotoninergic differentiation of 1C11 cells. These observations are in good agreement with the presence of 5-HT-2B mRNA in early mouse embryonic development. Furthermore, the major sites of 5-HT-2B mRNA embryonic expression are in the heart, and in the neural fold before the closure of the neural tube. Therefore, this receptor could account at least in part for the trophic functions attributed to the 5-HT-2-like receptors.