Mouse erythrocyte membrane sialoglycoproteins and their involvement in the interaction with mouse rosette forming human lymphocytes
Krotkiewski, H.; Daniluk, E.; Skibiński, G.; Lisowska, E.
Archivum Immunologiae et Therapiae Experimentalis 32(5): 613-620
1984
ISSN/ISBN: 0004-069X PMID: 6335962 Document Number: 231605
Sialoglycoproteins were isolated from Balb/c mouse erythrocyte membranes. The crude preparation showed 4 period acid-Schiff-positive zones in SDS-polyacrylamide gel electrophoresis. The lowest MW component was separated on the Ultrogel AcA44 column in the presence of SDS. The carbohydrate composition of the fractions obtained indicated that the sialoglycoproteins contained O-glycosidic oligosaccharide chains and that the lowest MW glycoprotein also contained N-glycosidic chain(s). The sialoglycoproteins strongly and specifically inhibited the rosette formation between mouse erythrocytes and human B lymphocytes. It is suggested that sialoglycoproteins are the mouse red cell components involved in the interaction with human lymphocytes.