Preparation of mouse antiserum against isologous aggregated immunoglobulins and its effect on rosette forming cells
Chernousova, L.N.; Kul'berg, A.I.; Simonian, S.A.; Iurin, B.L.; Khorobrykh, V.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 84(12): 698-700
1977
ISSN/ISBN: 0365-9615 PMID: 304364 Document Number: 121501
A method of obtaining antisera against isologous aggregated mouse immunoglobulins is described. This serum designated MAAS blocked in vitro the antigen-binding receptors of the immune rosette-forming cells. MAAS was injected to mice immunized with SRBC. In comparision with the immunized mice given normal isologous serum rosette-forming B-cells were absent in the spleen of mice given MAAS at the peak of isologous response. But the antibody-forming cell count was not decreased under the influence of MAAS.