Study of DNA-protein crosslinks induced by UV light in Hela cells
Kolomiĭtseva, G.I.; Kurochkina, L.P.; Bobkova, E.A.
Biokhimiia 59(8): 1192-1197
1994
ISSN/ISBN: 0320-9725 PMID: 7819405 Document Number: 433125
UV-Irradiation (lambda = 254 nm) of Hela cells in the monolayer causes a dose-dependent formation of DNA-protein crosslinks (DPC). The time-dependent protein increase unaccompanied by a change in the complex yield was observed for the fraction of the DNA-protein complex passing into the aqueous phase by phenol extraction, when the cells were transferred to a fresh culture medium after UV-irradiation. These data suggest that DPC do not repair, while DNA or the protein within the complex can firmly bind additional quantity of protein during the post-irradiation period.