Determination of total estrogen in urine with 3-methyl-2-benzothiazolinone hydrazone
Yee, H.Y.; Jackson, B.
Analytical Chemistry 48(12): 1704-1707
1976
ISSN/ISBN: 0003-2700 PMID: 970630 Document Number: 430939
An estrogen method was developed that utilizes a colorimetric reaction consisting of the oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) to the phenol portion of the steroid molecule. Colorimetric measurements are made at 530 nm manually or with an AutoAnalyzer at an analysis rate of 50 samples/h. Comparison with a fluorometric method gave a correlation coefficient of 0.96 and with a gas chromatographic one, a coefficient of 0.94, so that the proposed method may be suitable as an alternative method for the determination of total estrogens in pregnancy urine samples.