Evaluation of the new Cobas Core IgE Total EIA in comparison to three commercial IgE immunoassays
de Weck, A.L.; Schluchter, M.; Girardin, P.
Journal of Investigational Allergology and Clinical Immunology 4(1): 5-11
1994
ISSN/ISBN: 1018-9068 PMID: 8081542 Document Number: 430711
Determination of total IgE by the new Cobas Core IgE Total EIA was compared with three other routine methods (Pasteur IgE ELISA, Pasteur IgE RIA and Pharmacia IgE Ultra-PRIST). Mean IgE concentrations in adult sera correlated well among the three methods (Cobas Core IgE Total EIA, Pasteur IgE ELISA and Pasteur IgE RIA); however, the values for the Pasteur IgE RIA were on average 20-50 IU/ml higher than those measured by the enzyme immunoassays. The correlation between the Cobas Core IgE Total EIA and the Pharmacia IgE Ultra-PRIST in sera from newborns was also excellent. The analytical performance of the Cobas Core IgE Total EIA in terms of dilution linearity, recovery and intra-assay reproducibility (coefficient of variation = 1.8-3.8%) was excellent.