Effect of sera of cirrhotic patients with or without hepatitis B virus infection on protein synthesis in hepatoma cells
Hajnická, V.; Volná, A.; Stancek, D.; Oltman, M.
Acta Virologica 38(1): 55-57
1994
ISSN/ISBN: 0001-723X PMID: 7520665 Document Number: 430327
The in vitro effects of sera of 11 patients with liver cirrhosis on protein synthesis in PLC/PRF/5 cells were studied. Hepatitis B virus (HBV) infection was documented in 7 patients. Increased random production of several cell proteins of M(r) of approximately 25, 65, 90 and 130 K was shown by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). There was no correlation between HBV-positive and HBV-negative cirrhosis and the induced proteins. One of them was identified as alpha-1 foetoprotein by immunoblot analysis. C-reactive protein (CRP) was determined only in one case; production of interleukin-6 (IL-6) was not detected.