The effect of phenobarbital on the nonenzymatic glycation of hemoglobin
Niketić, V.; Bogavac, S.; Tomasević, N.
Farmaco 49(10): 659-662
1994
ISSN/ISBN: 0014-827X PMID: 7826473 Document Number: 427752
The effect of phenobarbital (PB) on nonenzymatic glycation of hemoglobin in vivo and in vitro was studied. In epileptic patients on long-term therapy with PB, the yield of glycated hemoglobin fraction HbA-1c was found to be significantly higher than in normal control. In samples of Hb exposed to glucose in vitro in the presence of PB, HbA-1c was also found to be significantly higher than in those exposed to glucose in the absence of PB. Enhanced reactivity of beta-NH-2 terminus of Hb with glucose, was partly explained by observing that its pK-a was decreased in the presence of PB. It was shown that the effect of PB on pK-a of beta-NH-2 terminus is one of the consequences of the Hb-PB interactions which were studied in this work by electrophoretic and NMR techniques.