Top Abstract Purchase Related
Home
>
Node
>
Subnode

MultiCycle software for cell cycle analyses

Funai, S.; Kurooka, K.; Fujii, Y.; Inufusa, H.; Yamada, H.; Imanishi, Y.; Sato, S.; Shindo, K.; Yasutomi, M.

Gan to Kagaku Ryoho. Cancer and ChemoTherapy 21(Suppl 1): 102-107

1994

ISSN/ISBN: 0385-0684
PMID: 8203921
Document Number: 423387
MultiCycle software (M-cycle), a computer cell cycle analysis program that has a background debris and aggregation compensating function, was utilized in this study to prove the usefulness of the M-cycle. The S phase fraction (SPF) calculated by the M-cycle was compared to that of bromodeoxyuridine labelling index (BLI) using colorectal carcinoma cell lines. The SPF value was slightly lower using the M-cycle than that of the BLI in Colo 201 and Colo 320 and lower significantly in Widr. This may indicate that the M-cycle effectively compensated for the background existing in the DNA histogram. The SPF value was computed both by the M-cycle and the sum of broadened rectangles model (SOBR). The SPF value of these cell lines showed a lower figure in the M-cycle than in the SOBR. The SPF value of paraffin-embedded material through the M-cycle and the SOBR was compared according to DNA ploidy patterns. The SPF value computed by the M-cycle was significantly lower in both ploidy patterns than that of the SOBR. In conclusion, the M-cycle is a useful tool for cell cycle analyses of simple DNA flow cytometric histograms obtained by paraffin-embedded material.

Document emailed within 1 workday
Secure & encrypted payments

Titus, P.H. 2000: Structural analyses, sensivity analyses, and independent confirmatory analyses of the IGNITOR magnet system Ieee Trans. Appl. Supercond 10(1): 641-644
Deysson, G.; Bonaly, J. 1970: Experimental modifications of the cellular cycle studied on the radicular meristem of Allium sativum L. I. Determination of the normal cell cycle Annales Pharmaceutiques Francaises 28(11): 605-614
Basnak'ian, I.A.; Miriasova, L.V.; Dubinina, G.P.; Shmeleva, E.I.; Mertsalova, N.U. 1981: Viability of pertussis bacteria in a multicycle cultivation process Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 103-105
Muller, F.; Aegerter, P.; Ngo, S.; Fort, A.; Beauchet, A.; Giraudet, P.; Dommergues, M. 1999: Software for prenatal down syndrome risk calculation: a comparative study of six software packages Clinical Chemistry 45(8 Part 1): 1278-1280
Ristic-Fira, A.; Nikolic, D.; Petrovic, I.; Ruzdijic, S.; Raffaele, L.; Sabini, M.G.; Cirrone, G.A.; Cuttone, G.; Farruggia, G.; Masotti, L.; Kanazir, D.T. 2001: The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line Journal of Experimental and Clinical Cancer Research: Cr 20(1): 135-143
Zhuang, L.; Guo, H.; Xin, D.; Zhang, Z.; Li, H.; Yuan, X.; Tang, D.; Ding, Y.; Liu, L.; Guo, Y. 2000: Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle Chinese Medical Journal 113(4): 306-309
Peng, L.; Liu, X. 2015: Software version and medical device software supervision Zhongguo Yi Liao Qi Xie Za Zhi 39(1): 48-50
Strakova, N.; Ehrmann, J.; Bartos, J.; Malikova, J.; Dolezel, J.; Kolar, Z. 2005: Peroxisome proliferator-activated receptors (PPAR) agonists affect cell viability, apoptosis and expression of cell cycle related proteins in cell lines of glial brain tumors Neoplasma 52(2): 126-136
Gushchin, V.A. 1984: Calculation of Steel's cell loss factor and of the cellular kinetic parameters for a population with an exponential growth of the cell number and an equal probability of cell death in all phases of the life cycle Tsitologiia 26(12): 1365-1371
Buell, D.N.; Fowlkes, B.J.; Metzher, H.; Isersky, C. 1976: Cell cycle and morphological changes during growth and differentiation of a rat basophilic leukemia cell line Cancer Research 36(9 Pt.1): 3131-3137
Brüsselbach, S.; Möhle-Steinlein, U.; Wang, Z.Q.; Schreiber, M.; Lucibello, F.C.; Müller, R.; Wagner, E.F. 1995: Cell proliferation and cell cycle progression are not impaired in fibroblasts and ES cells lacking c-Fos Oncogene 10(1): 79-86
Li, L.; Qi, F.-y.; Liu, J.-r.; Zuo, L.-f. 2005: Effects of ginsenoside Rh2(GS-Rh2) on cell cycle of Eca-109 esophageal carcinoma cell line Zhongguo Zhong Yao Za Zhi 30(20): 1617-1621
Nishimoto, T. 1995: Cell cycle analysis of temperature sensitive mutants for cell growth Human Cell 8(3): 89-94
Kurabe, N.; Arai, S.; Miyazaki, T. 2009: Cell cycle and cell sizing regulation via death effector domain Seikagaku. Journal of Japanese Biochemical Society 81(1): 24-27
Goroshkina, G.I.; Soĭfer, V.N. 1971: Effect of caffeine on the mitotic index and cell cycle duration in Hela cell cultures Tsitologiia 13(8): 1031-1034
He, Y.; Huang, Y.; Yang, F. 1992: Effects of fibronectin on cytodifferentiation and cell cycle in a human hepatoma cell line QGY-7703 Hua Xi Yi Ke da Xue Xue Bao 23(4): 381-385
Miyamoto, H.; Ito, M.; Araya, Y.; Takaoka, K.; Isobe, H.; Dosaka, H.; Inoue, S.; Kawakami, Y.; Mizuno, S. 1984: Etoposide effect on cell cycle kinetics of a human lung cancer cell line Gan to Kagaku Ryoho. Cancer and ChemoTherapy 11(6): 1237-1243
Duchrow, M.; Schlüter, C.; Key, G.; Kubbutat, M.H.; Wohlenberg, C.; Flad, H.D.; Gerdes, J. 1995: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins Archivum Immunologiae et Therapiae Experimentalis 43(2): 117-121
Zätterström, U.K.; Källén, A.; Wennerberg, J. 1991: Cell cycle time, growth fraction and cell loss in xenografted head and neck cancer In Vivo 5(2): 137-142
Hunáková, L.; Chorváth, M.; Duraj, J.; Bartosová, Z.; Sevcíková, L.; Suliková, M.; Chovancová, J.; Sedlák, J.; Chorváth, B.; Boljesíková, E. 2000: Radiation-induced apoptosis and cell cycle alterations in human carcinoma cell lines with different radiosensitivities Neoplasma 47(1): 25-31