Analysis of the conformational mobility of DNA in transcriptionally-active chromatin
Kraevskiĭ, V.A.; Krylov, D.I.; Razin, S.V.; Mikhaĭlov, V.S.
Biofizika 38(1): 108-116
1993
ISSN/ISBN: 0006-3029 PMID: 8471635 Document Number: 420934
An attempt is made to estimate the degree of conformational flexibility of DNA in different fractions of nucleosomes of transcriptionally active chromatin of higher eukaryotes, and in yeast nucleosomes as well. We have used the circular dichroism method to estimate conformational changes in DNA induced by temperature shift. The DNA conformational potential was shown to be approximately the same in all the fractions of nucleosomes under study.