Isolation and susceptibility to nucleases of transcriptionally active and inactive chromatin fractions from Physarum polycephalum
Czupryn, M.; Toczko, K.
Acta Biochimica Polonica 27(2): 143-151
1980
ISSN/ISBN: 0001-527X PMID: 7435080 Document Number: 165520
Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the labelled nascent RNA. Both chromatin fractions were shown to have the nucleosomal structure. DNA of the active fraction of chromatin was degraded much faster with DNAase I and micrococcal nuclease than the DNA of the inactive fraction.