Effect of protein kinase ck2 on topoisomerase i from plasmodia of the slime mold Physarum polycephalum
Derlacz, R.A.; Kowalska-Loth, B.; Staroń, K.
Acta Biochimica Polonica 45(3): 769-773
1998
ISSN/ISBN: 0001-527X PMID: 9918503 Document Number: 489785
Relaxing activity of Physarum topoisomerase I was increased by calf thymus protein kinase ck2, similarly as was the activity of mammalian topoisomerase I, despite a pronounced difference between amino-acid sequences of non-conserved domains of Physarum and mammalian enzymes. This feature of Physarum topoisomerase I was cancelled in nuclear extracts isolated from dibutyryl-cAMP treated plasmodia in which the activity of protein kinase ck2 was elevated.