Functional CD4 T cell subset interplay in an intact immune system
Murray, J.S.; Madri, J.; Pasqualini, T.; Bottomly, K.
Journal of Immunology 150(10): 4270-4276
1993
ISSN/ISBN: 0022-1767 PMID: 8097751 Document Number: 414572
Immunization with human collagen IV has been shown to lead to the selective activation of Th-1 and Th-2-like cells in vivo depending on the I-A genotype of the mice. Mice expressing I-A-b generate Th-1 cells, and mice expressing I-A-b generate Th-2 cells after immunization. We examined the response of (bxs)F1 hybrid mice to determine the types of CD4 T cell activated. We found that Th-1-like responses dominated, in that CD4 T cells from human collagen IV-primed mice displayed good proliferative responses and little ability to induce antibody formation, and secreted IFN-gamma and not IL-5. Most of the Th-1-like activity of F1 hybrid T cells was I-A-s restricted. Inhibition of Th-1 cell priming using anti-I-A-s antibody administered with the priming Ag in vivo revealed Th-2-like activity, in that the CD4 T cells now secreted IL-5 and not IFN-gamma and induced antibody formation. Additional studies indicated that anti-IFN-gamma treatment in vivo also revealed Th-2 cells, suggesting that I-A-s-restricted CD4 T cells producing or controlling the production of IFN-gamma suppress Th-2 priming.