Screening of synthetic peptide libraries with radiolabeled acceptor molecules
Kassarjian, A.; Schellenberger, V.; Turck, C.W.
Peptide Research 6(3): 129-133
1993
ISSN/ISBN: 1040-5704 PMID: 8318743 Document Number: 414393
A method has been developed for the identification of specific acceptor molecule-binding sequences from a chemically synthesized peptide library. The peptide resin beads, each bead carrying one peptide sequence, are incubated with radiolabeled acceptor molecule and subsequently immobilized in a thin layer of agarose. Resin beads that carry acceptor molecule-specific sequences are identified by autoradiography and subjected to automated gas-phase sequencing. The method was tested by screening a synthetic pentapeptide library with an anti-beta-endorphin monoclonal antibody.