A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen
Kutuzov, V.A.; Mikhaĭlov, V.V.; Kruglova, S.E.; Kutuzov, V.V.; Kirillov, A.P.; Krasnianskiĭ, V.P.; Pshenichnov, V.A.; Lebedinskaia, E.V.
Voprosy Virusologii 38(1): 17-18
1993
ISSN/ISBN: 0507-4088 PMID: 8073738 Document Number: 411775
A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.