Purification and properties of calmodulin from Phymatotrichum omnivorum
Sambandam, T.; Gunasekaran, M.
Microbios 73(294): 61-74
1993
ISSN/ISBN: 0026-2633 PMID: 8382768 Document Number: 408612
Mycelia of Phymatotrichum omnivorum obtained at 10 day intervals during 10 to 50 days of growth were used for isolating calmodulin, and studying its effect on glycogen synthetase, phosphorylase, phosphorylase kinase, cyclic AMP phosphodiesterase and Ca++ ATPase. Glycogen synthase was inhibited until the 30th day by calmodulin, whereas calmodulin obtained from the 40th day stimulated glycogen synthase activity and the 50th day sample had no effect. cAMP phosphodiesterase and Ca++ ATPase of P. omnivorum were stimulated by the respective calmodulin. Molecular weight of the purified fungal calmodulin was approximately 18 kD as revealed by sodium dodecyl sulphate gel electrophoresis. Trifluoperazine, dibucaine and lidocaine inhibited calmodulin activity and calmodulin activity and calmodulin activation of PDE, respectively.