Purification and properties of RNA polymerase from acidophilic heterotroph SJH
Beebee, T.J.
Biochemistry International 19(6): 1223-1229
1989
ISSN/ISBN: 0158-5231 PMID: 2635860 Document Number: 332624
RNA polymerase was isolated from the acidophilic heterotrophic bacterium SJH and purified to homogeneity. Subunit structure (.beta.',.beta.,.theta.,.alpha.2) resembled that of other gram-negative bacteria such as Escherichia coli. pH optimum of the enzyme was around 8.0 although the bacterium grew only below pH 6.0 and optimally between 3.0-4.0. SJH and E. coli RNA polymerases showed maximal activities on homologous high molecular-weight DNA templates.