The effect of primary BHV-1 infection on the dynamics of T and B lymphocytes in the peripheral blood and levels of specific serum antibodies in calves treated with glucan
Paulík, S.; Levkutová, M.; Bajová, V.; Benko, G.; Harvan, M.
Veterinarni Medicina 38(8): 477-483
1993
ISSN/ISBN: 0375-8427 PMID: 8236630 Document Number: 408366
In the present study we investigated the dynamics of circulating T and B lymphocytes and serum specific antibodies in calves experimentally infected with IBR virus (I group) and in calves administered glucan (seven days before infection) and the infected (GI group). The percentages of T and B lymphocytes in the peripheral blood were determined from analyses by rosette methods; the titer of serum anti-IBR antibodies was determined by virus-neutralizing test on cell cultures. The dynamics of the percentage of circulating T lymphocytes showed a similar decreasing trend in both groups, with significant values on days 3 to 5 after infection (AI). In comparison with the calves of I group, the outset of T cell reduction was found to be less pronounced (within the first two days after infection), with a statistically significant difference on day 2 AI (P < 0.05), Fig. 1. A decrease in the percentage of T lymphocytes was related to an increase in the percentage of circulating B lymphocytes, with maximum on days 3 and 4 AI (P < 0.05), Fig. 1. All the calves before experiment beginning were free of serum anti-BHV 1 antibodies. They started responding to the experimental infection with IBR virus by production of serum antibodies between week 1 and 2 AI. The dynamics of serum anti-IBR antibodies showed an identical course in both experimental groups, with the more pronounced outset (P > 0.05) of immunological response in the calves of GI group (Fig. 2).