In vitro primary immunization of human peripheral blood lymphocytes to KLH: evidence for HLA-D-region restriction
Rodey, G.E.; Luehrman, L.K.; Thomas, D.W.
Transplantation Proceedings 11(4): 1858-1859
1979
ISSN/ISBN: 0041-1345 PMID: 93813 Document Number: 140946
Human T . KLH-primed cells recovered from primary cultures were restimulated in a 2nd culture containing autologous or allogeneic monocytes in the presence or absence of KLH and proliferation was determined by incorporation of 3H-methyl-thymidine. Secondary proliferation of primed T cells required both autologous adherent cells and KLH in the cultures. Allogeneic adherent cells worked as effectively as autologous adherent cells in supporting proliferation only if the allogeneic cell shared an HLA-D region determinant in common with the autologous adherent cells used during the priming culture. Proliferative responses in cultures containing allogeneic adherent cells with no shared D-region specificities were markedly diminished or absent. This D region restriction was further confirmed by demonstrating that the addition to the secondary cultures of an anti-DR antiserum directed against the shared DR determinant abrogated proliferation, but anti-DR antisera against the nonshared DR determinants on primed T cell or allogeneic adherent cell did not. Additional evidence is provided that the genetic restrictions governing human cell interactions of in vitro immune responsiveness are analogous to those observed in other animal models. Probably these in vitro techniques can also be used to define Ir genes which control immune responsiveness to humans and ultimately to better characterize the HLA-linked genetic factors that contribute to the susceptibility to certain disease states that are associated with HLA-D region gene products.