The determination of leukocyte osmotic resistance in hypertonic solutions of low-molecular nonelectrolytes
Vavilov, M.P.; Apollonova, L.A.; Nazarova, E.K.
Klinicheskaia Laboratornaia Diagnostika 6: 40-43
1993
ISSN/ISBN: 0869-2084 PMID: 8032781 Document Number: 407165
A method for in vitro assessment of blood leukocyte osmotic stability has been developed. A characteristic feature of this method is assessment of qualitative and quantitative parameters in hypo- and hypertonic solutions of low-molecular nonelectrolytes characterized by erythrocytolytic properties (glycerol, urea, dimexide). The error is +/- 10%. The results of the method are compatible and reproducible, it gives sufficient volume of information about leukocyte osmotic stability as an integral indicator of these cells physicochemical properties. A parabolic type of osmotic stability relationship with concentrations in the 0.04 to 5.0 M range has been detected. The optimal concentrations at which the highest leukocyte intactness is observed are 2.0 M for glycerol, 0.5 M for urea, and 5.0 M for dimexide.